Total nutrition and protein content determination in colostrum and mature human milk by different methods
1986
Ten, A. | Pamblanco, M.
Extract: Two different spectrophotometric methods to estimate protein contents were compared in 123 samples of human milk corresponding to the first month postpartum from mothers of different gestational age and birthweight infants. In spite of the significant correlation coefficient found (r=0.575) between A280nm and protein content values, the separated analysis of the groups of samples has shown great variations. It is suggested that this relation could be used only for specific cases. The correlation between the spectrophotometric measures with the values obtained with an established method to determine total nitrogen (micro-Kjeldahl) results in r values of 0.685 and 0.487 for the methods of Flores and Nakai and Le, respectively. The method of Flores utilized on delipidated milk samples appears to be suitable to estimate protein and total nitrogen content in human milk.(author)
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