Purification of human respiratory syncytial virus fusion glycoprotein
2012
Zheng, Yanpeng | Tang, Yaning | Fu, Yuanhui | He, Jinsheng | Wang, Xiaobo | Hong, Tao
Human respiratory syncytial virus (RSV) fusion glycoprotein (F) elicits neutralizing antibodies to RSV and has therefore attracted much attention as a suitable candidate antigen in the development of gene-based vaccines against RSV infections. However, a major obstacle in vaccine development has been the problem of antigen purification. To address this problem, we have developed a new method that combines sucrose gradient ultracentrifugation and a two-step chromatographic process, to purify RSV F from RSV particles propagated in HEp-2 cells. Analysis of the fractions produced using this method showed recovery of a functional homodimer with a molecular weight of 140kDa, and 54% preservation of the original F.
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