Rapid and direct analysis of statins in human plasma by column-switching liquid chromatography with restricted-access material
2014
Fagundes, Vinicius Freire | Leite, Camila Prado | Pianetti, Gerson Antonio | Fernandes, Christian
This study presents the development of a column-switching liquid chromatographic method with direct injection of human plasma for simultaneous determination of four statins (lovastatin, pravastatin, rosuvastatin and simvastatin), the main class of drugs used in the treatment of hyperlipidemia. By using a C18 (30mm×4.6mm, 15μm) a lab made bovine serum albumin restricted access material (RAM) column was prepared and compared with a commercial alquil-diol silica RAM column (C18, 25mm×4.0mm, 25μm) in terms of their protein exclusion capacity and micromolecules retention. Foreflush and backflush modes were compared for both RAM columns to the number of theoretical plates, asymmetry, resolution and chromatographic run time. The developed method was validated in the range from 125 to 876ngmL−1 for lovastatin, rosuvastatin and simvastatin, and from 500 to 2000ngmL−1 for pravastatin, presenting selectivity, precision and accuracy intra and inter-run. Total analysis time (sample preparation and chromatographic separation) was only 16min when the backflush mode was employed in the column-switching system.
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