Effect of Fluorination Modification on Transfection Efficiency of Non-Viral Gene Carrier Systems Based on Chitosan
2019
GOK, Mehmet Koray
The aim of this study was to examine the transfection efficiency of the fluorination modification without the addition of any cationic charge on the chitosan (Chi) molecule. The fluorination reaction on Chi (ChiF) was carried out with using 1H,1H,2H,2H-Perfluorooctyltriethoxysilane (SiF). The characterization of ChiF was realized by Fourier transform infrared (FTIR) analysis and its molecular weight (Mw) and polydispersity index (PDI Mw) were determined using GPC-SEC system. The physical properties of nanoparticles (nChiF) obtained by ionic gelation method were determined. The gel electrophoresis analysis was applied to the nanoparticles for determine the gene complexing capacity. The cytotoxicity of ChiF onto Human Embryonic Kidney (HEK293) cells was determined via MTT colorimetric assay. The cell confluency (after transfection) and transfection efficiency of nChiF on HEK293 cells were evaluated. The results showed that the nChiF2:pEGFN1 complex (ratio of 35:1) with a particle size of 98.1+-2.2 nm and zeta potential of 34.7?+-?6.5?mV, is more superior agent for transfection efficiency in HEK293 cells due to its high transfection effect and higher cell confluency. As a result, it has been showed that the fluorination reaction onto Chi without any cationic charge modification enhance the transfection efficiency for HEK293 cell lines.
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