The survival of <I>Mycoplasma bovis</I> at different temperatures
2010
Vyletělová, M.
The survival of <I>Mycoplasma bovis</I> in milk samples was investigated at three storage temperatures (5°C, –30°C and –80°C) during 5 weeks. For the storage temperatures –30°C and –80°C, the respective samples were prepared weekly and those for culture by repeated defrosting. At 5°C the total number of <I>M. bovis</I> CFU/ml decreased from the initial of 1.13 × 10<sup>7</sup> CFU/ml to the final of 3.92 × 10<sup>6</sup> CFU/ml. The development in the frozen samples prepared for each week was as follow: (1) at –30°C – from the initial of 1.13 × 10<sup>7</sup> CFU/ml to 5.69 × 10<sup>6</sup> CFU/ml; (2) at –80°C – from the initial of 1.13 × 10<sup>7</sup> CFU/ml to 9.75 × 10<sup>6</sup> CFU/ml. The decrease in <I>M. bovis</I> colony count was more evident in the samples that were repeatedly defrosted: (1) at –30°C – the initial of 1.13 × 10<sup>7</sup> CFU/ml to the final of 2.18 × 10<sup>6</sup> CFU/ml; (2) at –80°C – the initial and final values were 1.13 × 10<sup>7</sup> CFU/ml and 7.89 × 10<sup>6</sup> CFU/ml, respectively.
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