Solid substrate bioassay to evaluate impact of Trichoderma on trichothecene mycotoxin production by Fusarium species
2008
Busko, Maciej | Chełkowski, Jerzy | Popiel, Delfina | Perkowski, Juliusz
BACKGROUND: Fusarium head blight of wheat is a destructive disease in various wheat-growing regions and leads to significant yield losses for farmers and to contamination of cereal grains with mycotoxins, mainly deoxynivalenol and its derivatives. Toxigenic Fusarium species sporulate on cereal crop residues and produce significant inoculum for epidemics. Reduction of mycotoxin production and pathogen sporulation may be influenced by saprophytic fungal antagonists.RESULTS: Trichoderma isolates were examined in dual culture bioassays on rice with two isolates of Fusarium graminearum Schwabe and two isolates of Fusarium culmorum (W.G. Smith) Saccardo, belonging to three different chemotypes. Production of five trichothecene mycotoxins, deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3AcDON), 15-acetyl-deoxynivalenol (15AcDON), nivalenol (NIV) and fusarenone X (FUS), was reduced by over 95%. Two Trichoderma isolates partially reduced the amounts of four metabolites when inoculated on autoclaved cultures of the same four Fusarium isolates. However, in the case of the 15AcDON chemotype of F. culmorum culture the content of DON increased and that of 15AcDON decreased. Isolates of Trichoderma varied in their ability to inhibit production of the five trichothecene mycotoxins by Fusarium. Susceptibility of the four Fusarium isolates to antagonistic activity of the same Trichoderma isolate differed significantly.CONCLUSION: Selected non-toxigenic Trichoderma isolates proved to be useful biocontrol agents against toxigenic Fusarium pathogens of wheat, significantly reducing production of the trichothecene mycotoxins DON, NIV and their acetylated derivatives.
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