Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment
2022
Homolak, Jan | Kodvanj, Ivan | Babic Perhoc, Ana | Virag, Davor | Knezovic, Ana | Osmanovic Barilar, Jelena | Riederer, P. (Peter) | Salkovic-Petrisic, Melita
We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.•NRP is a rapid, cost-effective, and simple method for reductive capacity assessment•NRP is compatible with a high-throughput screening of solid and liquid biological samples•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis
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