Exogenous NaHCO3 enhances growth and lipid accumulation of the highly NaHCO3-tolerant Nannochloris sp. JB17
2021
Wang, Min | Liu, Hua | Qiao, Kun | Ye, Xiaoxue | Takano, Tetsuo | Liu, Shenkui | Bu, Yuanyuan
The extremely saline-alkaline soil in the northeast of China is rich in Na₂CO₃ and NaHCO₃. Previously, we isolated the green alga Nannochloris sp. JB17 (JB17) from this soil and found that it was resistant to high concentrations of NaHCO₃. In this study, the biochemical characteristics of JB17 cultured with NaHCO₃ were studied by measuring growth and photosynthesis, and biomass, starch, and lipid production. The final cell density and maximum dry weight of JB17 cultured with 300 mM NaHCO₃ were 1.528 and 1040.8 mg L⁻¹, respectively, and were higher than those of JB17 cultured with 50 mM NaHCO₃ (control). The maximum chlorophyll-a content and oxygen evolution rate under 300 mM NaHCO₃ treatment were 26.5 mg g⁻¹and 182 μmol O₂ mg⁻¹ Chl h⁻¹, respectively, and were higher than those of the control. The addition of 300 mM NaHCO₃ increased the relative electron transfer rate (rETR) and maximum relative electron transfer rate of JB17. The total starch and lipids contents of JB17 significantly increased from 215 to 356 mg g⁻¹ and from 263 to 531 mg g⁻¹, respectively, after the addition of 300 mM NaHCO₃. Including NaHCO₃ in the culture medium greatly stimulated the growth, biomass accumulation, and lipid productivity of JB17. These results indicate that the accumulation of starch and lipids in JB17 cultured with NaHCO₃ might be related to the promotion of photosynthesis.
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