Transcriptome profiling analysis reveals involvement of SAM cycle and methionine pathway in low potassium tolerance in barley
2021
Ye, Zhilan | Zeng, Jianbin | Ma, Xinyi | Long, Lizhi | Zhang, Guoping
Available potassium in most agricultural soils is not sufficient for requirement of crop growth and development, and K deficiency has become a bottleneck of crop production worldwide. Development of low-K tolerant crop cultivars is an effective approach for relieving K deficiency. In the previous studies we identified some Tibetan annual wild barley accessions with higher low-K tolerance than cultivated barley. In this study, Illumina RNA-Sequencing was performed on the four-leaves seedlings of a Tibetan wild barley accession (XZ153, low-K tolerance) and a barley cultivar (ZD9, low-K sensitivity) to compare their transcriptome profiles in response to low-K stress. A total of 7263 differentially expressed genes (DEGs) were detected in the topmost 2 and 3 leaves (YL2, YL3) of the two genotypes under low-K stress, with XZ153 and YL2 having larger change than ZD9 and YL3, respectively, in terms of DEGs number and expression level. Meanwhile, 1395 low-K tolerance associated DEGs were mapped to metabolic process, translation, RNA methylation and responses to abscisic acid and other gene ontology (GO). The responses of XZ153 and ZD9 to low-K stress differed dramatically at the transcriptional level. The higher low-K tolerance in XZ153 is attributed to its more K uptake and accumulation in the topmost 2 leaf (YL2). The S-Adenosyl-l-methionine (SAM) cycle and methionine pathway involving in ethylene biosynthesis may account for the genotypic difference in low-K tolerance.
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