Broadening of genetic base in sugar crops : I. Genetic variability and diversity in induced haploids of sugarbeet
2001
Svirshchevskaya, A. | Srivastava, H. M.
Gynogenetic haploid and double haploid (DH) lines produced earlier from six Monogerm and multigerm sugarbeet genotypes at Minsk, Belarus, were used in the present study with the objectives to study genetic variability of induced haploids and doubled haploid in sugarbeet populations, and to demonstrate genetic diversity of gynogenetic germplasm with help of molecular markers. Afterin vitro period of induction and micropropagation, plants of gynogenetic origin were characterized by low values of root yield and sugar content, due to mixoploid tissues. Prebreeding field trials of 40 genotypes (lines, their hybrids and controls) showed wide variability in double haploids for quantitative traits, with higher values for root weight, top weight and sugar yield/root in DH lines compared to haploids and their controls. Genetic diversity and variability among DH lines of different origin alongwith checks was studied through AFLP techniques. The phylogentic tree for 19 selected genotypes was constructed which gave interesting information and six lines were selected as base material for broadening of genetic base to produce experimental hybrids.
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