Use of Fast Repetition Rate Fluorometry on Detection and Assessment of PAH Toxicity on Microalgae
2010
Pérez, Patricia | Fernández, Emilio | Beiras, Ricardo
Cultures of the flagellate Isochrysis galbana were used to carry out the ecotoxicological evaluation of four PAHs [(naphthalene, phenanthrene, pyrene (Pyr) and fluoranthene (Flu)] by monitoring growth rate and the fluorescence variables F ₀, F m, F v and F v/F m, determined with a fast repetition rate fluorometer. The results presented in this investigation showed that F v was a suitable endpoint in acute ecotoxicological tests with marine phytoplankton. The derived effective concentrations followed the known narcotic mechanism of toxicity and showed sensitivity levels comparable to marine invertebrate embryo-larval bioassays. Pyr and Flu showed the lowest EC₁₀, which ranged between 168-279 and 189-697 nM, respectively.
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