Detection and cellular location of cross-reactive antigens shared by Camellia sinensis and Bipolaris carbonum
1994
Chakraborty, B.N. | Saha, A.
Among the 14 tocklai varieties of tea tested against Bipolaris carbonum, TV-18 was found to be most susceptible and TV-26 most resistant under identical conditions. Antigens obtained from tea varieties, isolates of B. carbonum and nonpathogens of tea (Bipolaris tetramera and Bipolaris setariae), were compared by immunodiffusion, immunoelectrophoresis and enzyme-linked immunosorbent assay to detect cross-reactive antigens (CRA) shared by the host and the parasite. CRA were found among the susceptible varieties (TV-9, 17 and 18) and isolates of B. carbonum (BC-1, 2, 3 and 4). Such antigens were not detected between isolates of B. carbonum and resistant varieties (TV-16, 25 and 26), nonpathogens and tea varieties, as well as nonpathogens and isolates of B. carbonum. Indirect staining of antibodies using fluorescein isothiocyanate (FITC) indicated that in cross sections of leaves (TV-18), the CRA were concentrated mainly around epidermal cells. Treatment of mycelia and conidia of B. carbonum with antisera to leaves (TV-18) and indirect staining with FITC indicated the presence of CRA in the young growing hyphal tips and conidia.
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