Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids

Serror, P.; Sasaki, T.; Ehrlich, S.D.; Maguin, E.

Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids

2002

Serror, P. | Sasaki, T. | Ehrlich, S.D. | Maguin, E.

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per microgram of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii.

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Mots clés AGROVOC

bacteriophages classification electroporation electroporation genetic transformation genetic vectors genetics lactobacillus lactobacillus delbrueckii methods plasmid vectors plasmids transformation

Informations bibliographiques

Publié dans

Applied and environmental microbiology

Volume 68 Numéro 1 Pagination 46 - 52 ISSN 0099-2240

D'autres materias

Bacterial; Lactobacillus delbrueckii subsp. bulgaricus; Growth development; Virus integration; Replicon

Langue

anglais

Note

2019-12-04

Licence

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Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-29

Format: MODS

Fournisseur de données

Cette notice bibliographique a été fournie par National Agricultural Library

Découvrez la collection de ce fournisseur de données dans AGRIS

Liens

DOI http://dx.doi.org/10.1128/AEM.68.1.46-52.2002

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