In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells
2006
Lin, Y.L. | Wu, C.H. | Luo, M.H. | Huang, Y.J. | Wang, C.N. | Shiao, M.S. | Huang, Y.T.
The production of reactive oxygen species (ROS) is believed to be involved in liver injury and hepatic fibrosis. Activation of hepatic stellate cells (HSCs) is a key feature of liver fibrosis. Salvia miltiorrhiza is a traditional Chinese herb used in the treatment of cardiovascular and liver diseases to resolve stasis. The effects of salvianolic acid B (Sal B), a major component of Salvia miltiorrhiza, on oxidative damage include free radical DPPH scavenging, malondialdehyde (MDA) formation and ROS generation in primary rat hepatocytes and HSCs, and on α-SMA, and collagen expression in transforming growth factor-β1 (TGF-β1)-stimulated HSCs were examined. Results indicated that Sal B scavenged DPPH potently with an IC50 2.2 ± 0.2 μg/ml (3.06 ± 0.3 μM), inhibited lipid peroxidation and eliminated ROS accumulation in a concentration-dependent manner on primary rat hepatocytes and HSCs. Sal B also reduced α-SMA and collagen synthesis and deposition in HSCs, and had no direct cytotoxicity on both hepatocytes and HSCs. Our results suggest that Sal B ameliorated oxidative damage and eliminated ROS accumulation in hepatocytes, and attenuated HSC activation, potentially conferring hepatoprotective and anti-fibrogenic effects.
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