Somatic embryogenesis and plant regeneration from leaf callus of Hordeum vulgare
1988
Mohanty, B.D. | Ghosh, P.D.
Explants obtained from the basal portion of leaves of Hordeum vulgare (cv. Karan 92) gave rise to callus when cultured on Murashige and Skoog (MS) basal medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D). Initially, the callus was friable, shiny-white and watery but subsequently some compact, nodular callus appeared. The latter were cultured on MS medium containing 0.05 mg l−1 2, 4-D and 0.1 mg l−1 N6-furfurylaminopurine (kinetin), when plantlets were generated. Histological studies showed that plantlet regeneration occurred by the formation of somatic embryos. The regenerated plants had the normal diploid chromosome number (2n = 14).
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