Cellular distribution of transforming growth factor betas 1, 2, and 3 and their types I and II receptors during postnatal development and spermatogenesis in the boar testis
1997
Caussanel, V. | Tabone, E. | Hendrick, J.C. | Dacheux, F. | Benahmed, M.
Transforming growth factor betas (TGFbetas) 1, 2, and 3 and their types I and II receptors (TGFbetas RI and RII) were immunolocalized 1) during testicular development from the perinatal to the adult period and 2) in maturing germ cell populations at successive seminiferous epithelium stages. In the perinatal testis, TGFbeta isoforms and receptors were both preponderant in Leydig cells and in spermatogonia. At prepuberty, their expression appeared in Sertoli cells, while germ cells showed specific TGFbeta 1 and TGFbeta RI staining in the spermatocytes. In the adult testis, TGF beta ligands exhibited a preferential tubular distribution. TGFbeta 1 was mainly detected in young spermatocytes, TGFbeta 2 in Sertoli cells, and TGFbeta 3 in Sertoli and premeiotic germ cells. Although the two receptors were systematically observed together in various cells, our data indicate a predominance of one in comparison with the other depending on the cell type. TGFbeta RI was predominant in meiotic and differentiated germ cells and TGFbeta RII in somatic cells. Finally, in the adult testis, TGFbetas 1, 3, and RI showed a germ-cell pattern that depended upon the stage of the seminiferous epithelium cycle. Specifically, staining for the ligands was predominant before meiosis, and TGFbeta RI was present particularly during meiosis and spermiogenesis. Together, the temporal and spatial distribution of the TGFbeta system components suggests that these signaling molecules mar play a crucial role during specific steps of testicular development and during different waves of seminiferous epithelium maturation leading to spermatogenesis.
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