Target region sequencing and applications in plants
2021
Ostezan, Alexandra | McDonald, Samuel Clay | Tran, Dung Thuy | Souza, Renan Silva E | Li, Zenglu
Fine mapping quantitative trait loci (QTL) and understanding the molecular mechanism and underlying functions of genes are vital to the advancement of plant research and plant breeding. Target region sequencing (TRS) provides an efficient way to assist in accomplishing these goals. Once a genomic region is found to be responsible for a phenotype, TRS can be deployed to characterize that region. Understanding the available methodologies to perform TRS will be advantageous when determining how to proceed with specific projects. In this article, we reviewed and summarized four main methods that are being used for TRS in plants, including hybrid capture, PCR-based enrichment, DNA fragment circularization, and RNA capture. We also discussed the potential uses of these technologies in plant research, especially the marker discovery for breeding selection. The TRS techniques outlined in this review have the potential to become commonplace in plant research, thereby having a large impact.
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