Nanocall: an open source basecaller for Oxford Nanopore sequencing data
2017
David, Matei | Dursi, L. J. | Yao, Delia | Boutros, Paul C. | Simpson, Jared T.
Motivation: The highly portable Oxford Nanopore MinION sequencer has enabled new applications of genome sequencing directly in the field. However, the MinION currently relies on a cloud computing platform, Metrichor (metrichor.com), for translating locally generated sequencing data into basecalls. Results: To allow offline and private analysis of MinION data, we created Nanocall. Nanocall is the first freely available, open-source basecaller for Oxford Nanopore sequencing data and does not require an internet connection. Using R7.3 chemistry, on two E.coli and two human samples, with natural as well as PCR-amplified DNA, Nanocall reads have ∼68% identity, directly comparable to Metrichor ‘1D’ data. Further, Nanocall is efficient, processing ∼2500 Kbp of sequence per core hour using the fastest settings, and fully parallelized. Using a 4 core desktop computer, Nanocall could basecall a MinION sequencing run in real time. Metrichor provides the ability to integrate the ‘1D’ sequencing of template and complement strands of a single DNA molecule, and create a ‘2D’ read. Nanocall does not currently integrate this technology, and addition of this capability will be an important future development. In summary, Nanocall is the first open-source, freely available, off-line basecaller for Oxford Nanopore sequencing data. Availability and Implementation: Nanocall is available at github.com/mateidavid/nanocall, released under the MIT license. Contact: [email protected] Supplementary information: Supplementary data are available at Bioinformatics online.
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