Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola
2007
Chen, Wenhao | Xu, Chun-Ming | Zeng, Jian-Li | Zhao, Bing | Wang, Xiaodong | Wang, Yu-Chun
At appropriate concentrations, polyamines promoted the callus growth and echinacoside content of Cistanche deserticola while Ag⁺ increased the content of echinacoside and acteoside. In a 20-day culture period, when putrescine (25 μM) and Ag⁺ (10 μM) were added on day 8 and day 16, respectively, the echinacoside production (1.7 g l-¹) and acteoside production (0.4 g l-¹) reached the maximum, which were 1.4-fold and 1.5-fold of those in single putrescine treatment, 1.6-fold and 1.4-fold of those in single Ag⁺ treatment, respectively. Exogenous putrescine enhanced cell viability and antioxidant enzyme activity markedly, so increased the final biomass. Ag⁺ addition increased H₂O₂ content and phenylalanine ammonia-lyase activity significantly which led to higher echinacoside and acteoside contents.
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