Saccharomyces als Objekt für populations-genetische Versuche
2014
Gutz, H. | Emeis, C. C.
Microorganisms are suitable objects for experimental studies in population genetics. It is easy to cultivate large populations under exactly controlled conditions. But microorganisms reproduce usually vegetatively so that one factor is lacking which is of great importance in populations of higher organisms: The sexual propagation which implies a permanent rearrangement of the genes present.Saccharomyces is an unicellular ascomycet. The cells are diploid and propagate vegetatively by budding. Under certain conditions Saccharomyces is able to reproduce sexually by formation of ascospores. We have developed a simple technique which allows to enforce exclusively a sexual propagation in a yeast. Thus Saccharomyces is a suitable object for experiments in population genetics, in which the rearrangement of genes by sexuality has to be considered. The existence of a diploid phase makes it possible to work on problems resembling those in populations of higher organisms.In the present work methods are described for experimental work with yeast populations, and possibilities are which Saccharomyces offers for experiments in population genetics are discussed.
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