DNA structure associated with autonomously replicating sequences from plants
1989
Eckdahl, T.T. | Bennetzen, J.L. | Anderson, J.N.
DNA fragments capable of conferring autonomous replicating ability to plasmids in Saccharomyces cerevisiae were isolated from four different plant genomes and from the Ti plasmid of Agrobacterium tumefaciens. The DNA structure of these autonomously replicating sequences (ARSs) as well as two from yeast were studied using retardation during polyacrylamide get electrophoresis and computer analysis as measures of sequence-dependent DNA structures. Bent DNA was found to be associated with the ARS elements. An 11 bp ARS consensus sequence required for ARS function was also identified in the elements examined and was flanked by unusually straight structures which were rich in A + T content. These results show that the ARS elements from genomes of higher plants have structural and sequence features in common with ARS elements from yeast and higher animals.
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