Standardized preparation of single-cell suspensions from mouse lung tissue using the gentlemacs dissociator
2009
Jungblut, Melanie | Oeltze, Karen | Zehnter, Irene | Hasselmann, Doris | Bosio, Andreas
The preparation of single-cell suspensions from tissues is an important prerequisite for many experiments in cellular research. The process of dissociating whole organs requires specific parameters in order to obtain a high number of viable cells in a reproducible manner. The gentleMACS Dissociator optimizes this task with a simple, practical protocol. The instrument contains pre-programmed settings that are optimized for the efficient but gentle dissociation of a variety of tissue types, including mouse lungs. In this publication the use of the gentleMACS Dissociator on lung tissue derived from mice is demonstrated.
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