Testing the efficacy of broad-acting sorbents for environmental mixtures using isothermal analysis, mammalian cells, and H. vulgaris
2021
Wang, Meichen | Chen, Zunwei | Rusyn, Ivan | Phillips, Timothy D.
The hazards associated with frequent exposure to polycyclic aromatic hydrocarbons (PAHs), pesticides, Aroclors, plasticizers, and mycotoxins are well established. Adsorption strategies have been proposed for the remediation of soil and water, although few have focused on the mitigation of mixtures. This study tested a hypothesis that broad-acting sorbents can be developed for diverse chemical mixtures. Adsorption of common and hazardous chemicals was characterized using isothermal analysis from Langmuir and Freundlich equations. The most effective sorbents included medical-grade activated carbon (AC), parent montmorillonite clay, acid-processed montmorillonite (APM), and nutrient-amended montmorillonite clays. Next, we tested the ability of broad-acting sorbents to prevent cytotoxicity of class-specific mixtures using 3 mammalian in vitro models (HLF, ESD3, and 3T3 cell lines) and the hydra assay. AC showed the highest efficacy for mitigating pesticides, plasticizers, PAHs, and mycotoxins. Clays, such as APM, were effective against pesticides, Aroclors, and mycotoxins, while amended clays were most effective against plasticizers. Finally, a sorbent mixture was shown to be broadly active. These results are supported by the high correlation coefficients for the Langmuir model with high capacity, affinity, and free energy, as well as the significant protection of cells and hydra (p < 0.05). The protection percentages in 3T3 cells and hydra showed the highest correlation as suggested by both Pearson and Spearman with r = 0.84 and rho = 0.73, respectively (p < 0.0001). Collectively, these studies showed that broad-acting sorbents may be effective in preventing toxic effects of chemical mixtures and provided information on the most effective sorbents based on adsorption isotherms, and in vitro and aquatic organism test methods.
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