Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates
2012
Botcherby, Edward J. | Smith, Christopher W. | Kohl, Michael M. | Débarre, Delphine | Booth, Martin J. | Juškaitis, Rimas | Paulsen, Ole | Wilson, Tony
Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
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