An ESIPT-induced NIR fluorescent probe to visualize mitochondrial sulfur dioxide during oxidative stress in vivo
2021
Ren, Haixian | Huo, Fangjun | Wu, Xia | Liu, Xiaogang | Yin, Caixia
Based on the change in electron distribution of the benzopyrylium unit before and after sulfite addition, a 2-(2′-hydroxyphenyl)benzothiazole (HBT)-based fluorophore generated the excited state intramolecular proton transfer (ESIPT) process with a near-infrared enhanced emission at 836 nm and a large Stokes shift (286 nm). The probe was applied to image SO₂ derivatives in cells and mice. Our data will provide new ideas for the development of ESIPT-based fluorescence probe with longer wavelength emission.
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