Biochemical responses in mussels Perna perna exposed to diesel B5
2015
Nogueira, Lílian | Garcia, Danielly | Trevisan, Rafael | Sanches, Ana Letícia Madeira | da Silva Acosta, Daiane | Dafre, Alcir Luiz | Oliveira, Thiago Yukio Kikuchi | Almeida, Eduardo Alves de
In Brazil B5 blend (5% biodiesel and 95% diesel oil) has been adopted as mandatory fuel since 2010 for automotive vehicles. Since little is known about the effects of B5 exposure can promote on antioxidant system of marine biota this study aimed to assess if B5 can generate modifications in antioxidant parameters of mussels Perna perna. To address this question mussels were exposed to two concentrations of B5 (0.01mLL−1 and 0.1mLL−1) for 6h, 12h, 48h and 168h. Then the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and glutathione reductase (GR) were evaluated in gills and digestive gland as well as the contents of glutathione (GSH) and lipid peroxidation by measuring the malondialdehyde concentration (MDA). In the gills, GST activity decreased after 48h and GR after 12h of exposure to B5. In digestive glands, the activities of SOD, GPx and GR were changed due to treatments. GSH concentration increased in digestive gland after 6h and 12h and in gills after 48h for B5 0.1mLL−1 and after 168h in the digestive gland for B5 0.01mLL−1 treatment. No lipid peroxidation was detected. The integrated biomarker response index (IBR) evidenced a B5 effect in the digestive gland after 168h of exposure. Regarding the experimental conditions and species used in this study, long-term exposure to B5 is apparently more likely to affect the parameters tested in P. perna mussels.
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