Claudin-8 expression in Sertoli cells and putative spermatogonial stem cells in the bovine testis
2014
McMillan, Mary | Andronicos, Nicholas | Davey, Rhonda | Stockwell, Sally | Hinch, Geoff | Schmoelzl, Sabine
Adhesion molecules are expressed by both adult and embryonic stem cells, with different classes of adhesion molecules involved in cell-membrane and intercellular contacts. In this study the expression of the adhesion molecule claudin-8 (CLDN8), a tight-junction protein, was investigated as a potential marker for undifferentiated spermatogonia in the bovine testis. We found that CLDN8 was expressed by both spermatogonia and a subset of Sertoli cells in the bovine testis. We also showed co-expression of GFRα1 in testis cells with CLDN8 and with Dolichos biflorus agglutinin–fluorescein isothiocyanate (DBA–FITC) staining. We observed co-enrichment of spermatogonia and CLDN8-expressing Sertoli cells in DBA–FITC-assisted magnetic-activated cell sorting (MACS), an observation supported by results from fluorescence-activated cell sorting analysis, which showed CLDN8-expressing cells were over-represented in the MACS-positive cell fraction, leading to the hypothesis that CLDN8 may play a role in the spermatogonial stem-cell niche.
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