Insecticidal Activity and Chemical Composition of Essential Oil from Artemisia Judaica L. Against Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae)
2012
Abd-Elhady, Hany
The insecticidal properties of essential oil derived via the hydro-distillation method from aerial parts of Artemisia judaica L. were tested against the cowpea weevil, Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae). The repellent activity assay of essential oil against C. maculates adults indicated that in concentrations of 63.7, 31.9, 15.9, 8.0 or 4.0 μg/cm², the oil reduced egg laying by 92.5, 86.0, 61.8, 42.7 and 12.5%, respectively. Also, the residual-film assay showed that after 72 hours of treatment, concentrations of 50 and 40% were highly effective against the C. maculates adults. The sub-lethal effects of essential oil were investigated on fecundity and F₁ progany by exposing adult females to treated seeds. Both LC₂₅ and LC₅₀ of essential oil significantly reduced F₁ progany production compared to the control. The chemical composition of the essential oil was analyzed by GC-MS and the resulting oil piperitone (32.4%), camphor (20.6%) and (E)-ethyl cinnnamate (8.2%) were found to contain the major constituents of the oil. This provided the insecticidal properties of the essential oil against cowpea weevil.
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