Patterns of accumulation of Bean common mosaic virus in Phaseolus vulgaris genotypes nearly isogenic for the I locus
2006
Cadle-Davidson, M.M. | Jahn, M.M.
The I locus of Phaseolus vulgaris is genetically and phenotypically well described, conferring incompletely dominant, temperature-dependent resistance against viruses currently assigned to at least four Potyvirus species. Despite the fact that the resistance allele at this locus, the I gene, has been incorporated into nearly all bean germplasm worldwide, little is known regarding its resistance mechanism. In the present study, P. vulgaris lines nearly isogenic for I were challenged with Bean common mosaic virus (BCMV; genus Potyvirus) in order to investigate at the cellular level the temperature-dependent resistance reaction. Immunolocalisation and confocal laser scanning microscopy were employed to visualise the virus and to identify patterns of BCMV accumulation in resistant, susceptible and heterozygous genotypes. Virus was detected in all three genotypes regardless of temperature, supporting previous findings that BCMV accumulates in protoplasts containing the I allele. Genotype-specific and temperature-specific patterns of virus accumulation suggested a resistance mechanism that depends on host recognition of viral replication and/or local movement.
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