Pentadecaibins I–V: 15-Residue Peptaibols Produced by a Marine-Derived Trichoderma sp. of the Harzianum Clade
2021
van Bohemen, Anne-Isaline | Ruiz, Nicolas | Zalouk-Vergnoux, Aurore | Michaud, Aurore | Robiou du Pont, Thibaut | Druzhinina, Irina | Atanasova, Lea | Prado, Soizic | Bodo, Bernard | Meslet-Cladiere, Laurence | Cochereau, Bastien | Bastide, Franck | Maslard, Corentin | Marchi, Muriel | Guillemette, Thomas | Pouchus, Yves François
In the course of investigations on peptaibol chemodiversity from marine-derived Trichoderma spp., five new 15-residue peptaibols named pentadecaibins I–V (1–5) were isolated from the solid culture of the strain Trichoderma sp. MMS1255 belonging to the T. harzianum species complex. Phylogenetic analyses allowed precise positioning of the strain close to T. lentiforme lineage inside the Harzianum clade. Peptaibol sequences were elucidated on the basis of their MS/MS fragmentation and extensive 2D NMR experiments. Amino acid configurations were determined by Marfey’s analyses. The pentadecaibins are based on the sequences Ac-Aib¹-Gly²-Ala³-Leu⁴-Aib/Iva⁵-Gln⁶-Aib/Iva⁷-Val/Leu⁸-Aib⁹-Ala¹⁰-Aib¹¹-Aib¹²-Aib¹³-Gln¹⁴-Pheol¹⁵. Characteristic of the pentadecaibin sequences is the lack of the Aib-Pro motif commonly present in peptaibols produced by Trichoderma spp. Genome sequencing of Trichoderma sp. MMS1255 allowed the detection of a 15-module NRPS-encoding gene closely associated with pentadecaibin biosynthesis. Pentadecaibins were assessed for their potential antiproliferative and antimicrobial activities.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS