Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells

Wang, M.J.; Bokern, M.; Boehme, C.; Jones, K.C.; Harms, H.

Phytotoxicity, uptake and metabolism of 1,4-dichlorobenzene by plant cells

1996

Wang, M.J. | Bokern, M. | Boehme, C. | Jones, K.C. | Harms, H.

Phytotoxicity, uptake, and metabolism of 1,4-dichlorobenzene (1,4-DCB) by carrot (Daucus carota L.), soybean (Glycine max. L.), tomato (Lycopersicon esculentum Mill.), and red goosefoot (Chenopodiun rubrum L.) cell suspension cultures were studied. Sealed glass systems were utilized for the investigation because 1,4-DCB is volatile. The sealed systems effect the growth of plant cells, but do not provide different results when testing xenobiotic uptake and metabolism. 1,4-Dichlorobenzene (40 microgram in 40 ml medium was taken up by carrot (49%), soybean (50%), and red goosefoot (62%) cells. Only the soybean cell cultures provided evidence of the existence of metabolites of this compound, probably conjugates of chlorophenols. Conditions for phytotoxicity tests were modified because the growth of cell cultures was affected when sealed for longer than 2 d. 1,4-Dichlorobenzene is toxic to cell cultures of the three tested plant species (tomato, soybean, and carrot). Concentrations of 0.5 mM caused 50% growth inhibition in carrot and soybean culture. The tomato cultures were more sensitive, with 0.05 mM causing 50% growth inhibition.

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