Quantification of specific E. coli in gut mucosa from Crohn's disease patients
2011
Jensen, Stina Rikke | Fink, Lisbeth Nielsen | Struve, Carsten | Sternberg, Claus | Andersen, Jens Bo | Brynskov, Jørn | Nielsen, Ole Haagen | Brix, Susanne
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
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