Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles
2011
Dinçkaya, Erhan | Kınık, Özer | Sezgintürk, Mustafa Kemal | Altuğ, Çağrı | Akkoca, Aylin
The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K₃[Fe(CN)₆]/K₄[Fe(CN)₆] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1–14ng/mL with a standard deviation of ±0.36ng/mL. Finally, the biosensor was applied to a series of real milk samples.
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