Comparison of Sclerotinia minor Inocula for Differentiating the Reaction of Peanut Genotypes to Sclerotinia Blight
2006
Faske, T. | Melouk, H.A. | Payton, M.E.
Efficacy of four types of Sclerotinia minor inocula were compared for disease incidence, lesion development, and differentiating a disease response on susceptible and moderately resistant peanut genotypes. Inocula were evaluated on intact and detached peanut main stem tissue. Three inocula lacked an exogenous nutrient source, which included germinating sclerotia produced on peanut stem, 3-day-old dry mycelia, and perlite granules coated with fresh mycelial fragments. Inoculum with an exogenous nutrient source consisted of an agar plug with mycelium. The mycelial agar plug inoculum resulted in a greater disease incidence and a longer mean lesion length/day compared to other inocula on intact stem or detached stem tissue. All inocula produced longer (P < 0.05) lesions 6 d after inoculation on intact stem tissue for susceptible control and shorter lesions on three of the four moderately resistant genotypes. All inocula produced longer (P < 0.05) lesions 6 d after inoculation on detached stem tissue for moderately resistant cultivars compared to susceptible check. Although, all inocula resulted in similar lesion development differentials of susceptible and moderately resistant genotypes, mycelial agar plug inoculum had a consistent disease incidence for both intact stem and detached stem tissue.
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