Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction

Nash, J.W.; Hanson, L.A.; St Cyr Coats, K.

Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction

1995

Nash, J.W. | Hanson, L.A. | St Cyr Coats, K.

Bovine immunodeficiency virus (BIV) is prevalent in beef and dairy cattle, yet the mode(s) of BIV transmission are undefined. Using polymerase chain reaction, which specifically targeted a 235-bp, highly conserved region of the BIV pol gene, BIV-infected leukocytes were detected in the blood and milk of BIV-seropositive cows. These data confirm the presence of BIV in milk and identify the potential for lactogenic transmission of this virus.

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Mots clés AGROVOC

analysis animals blood blood bovine bovine immunodeficiency virus cattle cattle cells cytology diagnosis disease transmission dna genetics leukocytes leukocytes methods milk milk polymerase chain reaction polymerase chain reaction proviruses virology

Informations bibliographiques

Publié dans

American journal of veterinary research

ISSN 0002-9645

Editeur

British Arachnological Society

D'autres materias

Dna primers; Base sequence; Lung; Veterinary; Sequence homology; Nucleic acid; Viral; Isolation & purification; Cattle diseases; Consensus sequence; Immunodeficiency virus; Enzyme-linked immunosorbent assay; Molecular sequence data; Female; Lentivirus infections; Cultured

Langue

anglais

Note

2019-12-05

Type

Journal Article; Text

Sur AGRIS depuis: 2024-02-29

Format: MODS

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