Survey of bacteria associated with western corn rootworm life stages reveals no difference between insects reared in different soils
2019
Ludwick, Dalton C. | Ericsson, Aaron C. | Meihls, Lisa M. | Gregory, Michelle L. | Finke, Deborah L. | Coudron, Thomas A. | Hibbard, Bruce E. | Shelby, Kent
Western corn rootworm (Diabrotica virgifera virgifera LeConte) is a serious pest of maize (Zea mays L.) in North America and parts of Europe. With most of its life cycle spent in the soil feeding on maize root tissues, this insect is likely to encounter and interact with a wide range of soil and root rhizosphere microbes. Our knowledge of the role of microbes in pests and in agricultural fields with regards to pest management and plant health remains incomplete. An important component is to know which microorganisms are present that could play a role in life history or management. For this study, insects were reared in either autoclaved soil or soil from a geographically separate location that had not been autoclaved. Insects were sampled to determine the possible core bacteriome. Additionally, soil was sampled at each life stage to determine the contribution of soil to the rootworm bacteriome. We analyzed the V4 hypervariable region of bacterial 16S rRNA genes with Illumina MiSeq to survey the different species associated with the insect and the soils. Significant differences were noted between soil and insects with principal coordinate analysis. No significant differences existed between the insects reared on soils which did or did not undergo the autoclave process, or between non-diapausing and diapausing insects. The bacteria present in immature stages and in male and female adults revealed a possible core bacteriome of approximately 16 operational taxonomic units (OTUs).
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