Synergistic Catalysis of Glycosyltransferase and Sucrose Synthase to Produce Isoquercitrin Through Glycosylation of Quercetin
2019
Pei, Jian-jun | Chen, An-na | Zhao, Lin-guo | Cao, Fu-liang | Li, Xiang-qian | Xiao, Wei
Isoquercitrin exhibits a number of biological properties and has a higher water solubility than quercetin. In this work, an Arabidopsis glycosyltransferase with high activity on quercetin (AtUGT78D2) was coupled with an efficient quercetin-tolerant Glycine max sucrose synthase (GmSUS) to synthesize isoquercitrin by regenerating UDP-glucose from UDP. The important factors for optimal synergistic catalysis were determined. Through the use of a fed-batch operation, the final titer of isoquercitrin increased to 3830 mg/L with a corresponding molar conversion of 94.3% and a maximum number of UDP-glucose regeneration cycles (RCₘₐₓ) of 33.
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