NIR detection of honey adulteration reveals differences in water spectral pattern
2016
Bázár, György | Romvári, Róbert | Szabó, András | Somogyi, Tamás | Éles, Viktória | Tsenkova, Roumiana
High fructose corn syrup (HFCS) was mixed with four artisanal Robinia honeys at various ratios (0–40%) and near infrared (NIR) spectra were recorded with a fiber optic immersion probe. Levels of HFCS adulteration could be detected accurately using leave-one-honey-out cross-validation (RMSECV=1.48; R2CV=0.987), partial least squares regression and the 1300–1800nm spectral interval containing absorption bands related to both water and carbohydrates. Aquaphotomics-based evaluations showed that unifloral honeys contained more highly organized water than the industrial sugar syrup, supposedly because of the greater variety of molecules dissolved in the multi-component honeys. Adulteration with HFCS caused a gradual reduction of water molecular structures, especially water trimers, which facilitate interaction with other molecules. Quick, non-destructive NIR spectroscopy combined with aquaphotomics could be used to describe water molecular structures in honey and to detect a rather common form of adulteration.
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