Flow-through, viral co-infection assay for resource-limited settings
2015
Cretich, Marina | Torrisi, Marcello | Daminelli, Serena | Gagni, Paola | Plavisch, Lauren | Chiari, Marcella
Here we present a new and rapid immunofiltration assay for simultaneous detection of HIV p24 and hepatitis B virus antigens. The assay platform is composed of a 13mm nitrocellulose filter spotted with capturing bioprobes and inserted in a Swinnex® syringe filter holder. Samples and reagents are flown through the nitrocellulose filter by manual pressure on the syringe. A colorimetric detection allows for naked eye results interpretation. The assay provides sensitivity in the picomolar range in just 5min, even using low volumes of sample in complex matrix. Probe deposition by spotting allows for flexible combinations of different capturing agents and multiple diagnoses; furthermore, the very simple and inexpensive set-up makes the syringe-based immunoassay on paper microarray a suitable diagnostic system for resource-limited settings.
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