Transcriptomic analysis reveals potential pathways associated with salt resistance in pecan (Carya illinoensis K. Koch)

Soil salinity is a serious abiotic stress worldwide. Pecan plants (Carya illinoensis K. Koch) have been suggested for cultivation in soils with high levels of salinity owing to their huge demand. To understand the potential molecular mechanisms in pecan in response to salt stress, RNA-sequencing technology was used to compare the transcriptomes of pecan plants treated with 0, 0.3 %, or 0.6 % NaCl solutions. The results indicated that 170,086 unigenes were obtained from pecan leaf cDNA samples. Based on the assembled de novo transcriptome, 53, 535, and 7358 differentially expressed genes (DEGs) were detected between untreated and salt-treated leaves at 8, 24, and 48 h, respectively. Because of the large number of DEGs across different contrasts, a Gene Set Enrichment Analysis was selected to identify gene pathways associated with salt treatment. A total of 1858 DEGs were enriched in 66 gene sets, including 22 up-regulated and 47 down-regulated gene sets in the salt treatment groups, compared with those in the control groups. The up-regulated gene sets were mainly involved in the response to salicylic acid; the regulation of the jasmonic acid-mediated signalling pathway during the short-term treatment (8 h); and the cellular response to hypoxia, cellular respiration, and RNA modification during the long-term treatment (24–48 h). The down-regulated gene sets were predominately associated with photosynthesis, water transport, and the metabolic biosynthetic process under salt stress. Genes related to the Really Interesting New Gene superfamily protein and F-box domain protein in the ubiquitin-dependent degradation pathway were significantly up-regulated or down-regulated in different periods of the regulating process. Overall, these results not only enrich genomic resources but also provide insights into the molecular mechanism in pecan under salt stress.