A BAC-based physical map of the major autosomes of Drosophila melanogaster
2000
Hoskins, R.A. | Nelson, C.R. | Berman, B.P. | Laverty, T.R. | George, R.A. | Ciesiolka, L. | Naeemuddin, M. | Arenson, A.D. | Durbin, J. | David, R.G.
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
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