Fruit Maturity and Quality of Splice-grafted and One-cotyledon Grafted Watermelon

Splice grafting with both cotyledons removed from the rootstock may significantly increase watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] grafting efficiency, eliminate rootstock regrowth, and reduce costs of watermelon transplant production. We evaluated the efficacy of antitranspirant and sucrose treatments on the survival of splice-grafted transplants and assessed the effects of grafting method and rootstocks on fruit yield and quality. First, in a greenhouse experiment, four commercial antitranspirants, applied to rootstock seedlings before splice grafting, increased transplant survival 21 days after grafting (DAG) from 7% to 35% to 68% (P < 0.0001). In a second greenhouse experiment, survival of splice-grafted seedlings was 91% for plants that received 2% sucrose solution + antitranspirant, compared with 67% for plants receiving 2% sucrose alone and 25% for plants that received only water (P < 0.0001). Finally, in a field experiment we compared splice- vs. one-cotyledon grafting with two rootstocks (‘Shintosa Camelforce’ and ‘Tetsukabuto’) vs. nongrafted plants. At 54 days after transplanting (DAT), survival of all grafted transplants averaged 96% with a plant vigor rating of 7.7/10 (10 = most vigorous), compared with 84% survival (5.8/10 vigor rating) for nongrafted transplants. Flowering was delayed by an average of 2 days for splice-grafted watermelon (37 DAT) vs. one-cotyledon grafted and nongrafted plants (P < 0.0001), but harvest date was the same for all treatments (70 DAT). Fruit were harvested 0, 7, and 14 days after fruit reached physiological maturity, and there was no difference in total yield or fruit quality between grafted and nongrafted treatments, with two exceptions. Fruit with splice-grafted ‘Shintosa Camelforce’ rootstock had the firmest flesh (8.2 N) compared with nongrafted transplants (5.3 N), and lycopene increased from 16.7 µg·g⁻¹ at physiological maturity to as high as 31.4 µg·g⁻¹ when harvested 7 days after physiological maturity (P = 0.0002). These results indicate that application of sucrose with antitranspirant to rootstock seedlings before grafting can increase the survival of splice-grafted watermelon, and splice-grafted watermelon perform similarly to one-cotyledon grafted and nongrafted watermelon plants in field production.