Preparation of soluble dietary fibers from Gracilaria lemaneiformis and its antitumor activity in vivo
2019
Ji, Hai-yu | Yu, Juan | Dong, Xiao-dan | Liu, An-jun
Gracilaria lemaneiformis is one of the red seaweed in Gracilariaceae family native to China, and the primary structural characterization and in vivo antitumor activity of soluble dietary fibers (SDFs) extracted with 1.5 M NaOH solution from Gracilaria lemaneiformis were evaluated in this study. Our results indicated that Gracilaria lemaneiformis was an excellent source for the production of dietary fiber. The main composition and physicochemical properties analysis revealed that the obtained crude dietary fibers (cDFs) contained about 11.93% moisture, 6.09% ash, 0.95% fat, 5.14% protein and 73.32% dietary fiber (containing 12.57% soluble dietary fiber), and their swelling force, water holding capacity and cation exchange capacity were 13.10 mL/g, 5.97 g/g and 0.23 mmol/g, respectively. In addition, the results of FT-IR, GC, HPGPC, XRD and SEM indicated that SDFs were mainly composed of galactose with α-type glycosidic linkages and fibrous structure, and their average molecular weight was 20,958 Da. The in vivo antitumor tests revealed that SDFs could effectively suppress the growth of transplanted S180 tumors in Kunming mice. Besides, the spleen/thymus indexes, splenic lymphocytes proliferation, and NK cells activities, as well as the levels of serums IL-2, IFN-γ and TNF-α of S180-bearing mice were remarkably improved by SDFs oral administration. Our results suggested that SDFs had the potential to prevent and cure for cancers as a novel dietary supplement.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par National Agricultural Library
Découvrez la collection de ce fournisseur de données dans AGRIS