Evaluation of the anti-oxidant property and cytotoxic potential of the metabolites extracted from the bacterial isolates from mangrove Forest and saltern regions of South India
2018
Prathiba, Subramanian | Jayaraman, Gurunathan
The objective was to evaluate the anticancer and antioxidant activities of the methanolic extracts of halophilic bacteria, isolated from soil samples of Marakkanam saltern and Pichavaram mangrove forest, India. Radical Scavenging activity, reducing power, and metal ion chelation ability was used to evaluate the antioxidant potential of the metabolic extracts, whereas cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The methanolic extract of Bacillus VITPS7 exhibited significant antioxidant property. Bacillus VITPS14 and Bacillus VITPS16 extracts were cytotoxic against HeLa cell lines but not to A549 cell lines. Colorimetric assays for the presence of specific metabolites including, total flavonoid and β carotene content were performed. The presence of these specific classes of metabolites was confirmed by UV–Visible spectrophotometry, Nuclear Magnetic Resonance (NMR) spectroscopy and Gas Chromatography–Mass Spectrometry (GC–MS). Specific NMR signals revealed the presence of aromatic and unsaturated metabolites whereas GC–MS analysis indicated the presence of metabolites such as squalene and methyl hexadeconate. The present study thus reports for the first time the presence of squalene in Bacillus VITPS12 and Planococcus maritimus VITP21, in addition to other metabolites that contribute to the observed antioxidant or/and cytotoxicity, thus revealing the therapeutic potential of these selected halophilic bacterial isolates.
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