24‐Methyl/methylene sterols increase in monoxenic roots after colonization by arbuscular mycorrhizal fungi
2004
Fontaine, J. | Grandmougin‐Ferjani, A. | Glorian, V. | Durand, R.
• Characteristic sterols of transformed carrot (Daucus carota) and chicory (Cichorium intybus) roots colonized by different strains of arbuscular mycorrhizal (AM) fungi were identified. • Sterols were extracted, analysed and identified by gas chromatography/mass spectrometry (GC‐MS) from monoxenic cultures of mycorrhizal and nonmycorrhizal roots. After colonization by Glomus intraradices, Glomus proliferum and Glomus sp., carrot and chicory roots exhibited a significantly higher 24‐methyl/methylene sterol content. A correlation was established between the content of the sum of 24‐methyl cholesterol, 24‐methylene cholesterol and 24‐methyl desmosterol. • This study clearly established that the increment of these characteristic sterols is an appropriate indicator of colonization by AM fungi of transformed roots. • Metabolic origin and specificity of these sterols in mycorrhizal roots was researched. The 24‐methyl/methylene sterol increase was observed only when the interaction between fungus and plant was completely established and the fungus was present inside the roots.
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