Changes in the catechin and epicatechin content of grape seeds on storage under different water activity (aw) conditions
2007
Hatzidimitriou, E. | Nenadis, N. | Tsimidou, M.Z.
Storage effect on antioxidant content and capacity of grape seeds under different aw conditions (aw 0.33; 0.53; 0.75/50 days, 25 °C) was examined. Total phenol content (determined by the Folin-Ciocalteu method) decreased during storage though changes were trivial for samples stored at 33% or 53% RH. High level of humidity (75%) accelerated degradation and resulted in approximately 50% reduction of total phenol content. Minor loss of the DPPH radical scavenging activity (%RSA) of the extracts was observed. Catechin and epicatechin content monitored by RP-HPLC was reduced during storage, particularly at 75% RH. Epicatechin content proved to be less sensitive to water activity conditions than catechin content. Results of various in vitro assays (Folin-Ciocalteu, FRAP, DPPH, ABTS, CBA, ORAC and copper induced liposome oxidation) did not support difference in terms of resistance to oxidation. Based on the continuous release of gallic acid, our finding was related to hydrolytic reactions. Control of aw of grape seeds can be of practical importance for the wine industry.
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