Gene expression in coffee
2020
Marraccini, Pierre
Coffee is cultivated in more than 70 countries of the intertropical belt where it has important economic, social and environmental impacts. As for many other crops, the development of molecular biology technics allowed to launch research projects for coffee analyzing gene expression. In the 90s decade, the first expression studies were performed by Northern-blot or PCR, and focused on genes coding enzymes of the main compounds (e.g., storage proteins, sugars, complex polysaccharides, caffeine and chlorogenic acids) found in green beans. Few years after, the development of 454 pyrosequencing technics generated expressed sequence tags (ESTs) obviously from beans but also from other organs (e.g., leaves and roots) of the two main cultivated coffee species, Coffea arabica and C. canephora. Together with the use of real-time quantitative PCR, these ESTs significantly raised the number of coffee gene expression studies leading to the identification of (1) key genes of biochemical pathways, (2) candidate genes involved in biotic and abiotic stresses as well as (3) molecular markers essential to assess the genetic diversity of the Coffea genus, for example. The development of more recent Illumina sequencing technology now allows large-scale transcriptome analysis in coffee plants and opens the way to analyze the effects on gene expression of complex biological processes like genotype and environment interactions, heterosis and gene regulation in polypoid context like in C. arabica. The aim of the present review is to make an extensive list of coffee genes studied and also to perform an inventory of large-scale sequencing (RNAseq) projects already done or on-going.
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