Structural characterization of cytotoxic exopolysaccharides produced by Bifidobacterium pseudocatenulatum ATCC 27919 cultivated in aloe vera medium
2022
Khalilah Abd Khalil | Hifa Nadzirah Mohd Yaziz | Jeganathan Tharshan Jeyakanesh | Husna Zulkipli | Fatimah Salim
Exopolysaccharides (EPS) produced by several probiotics, including Bifidobacteria strains, have various benefits, such as anticancer activity due to its cytotoxic property components. However, different media have an influence of the components of EPS produced during cultivation and lead to differences in benefits and functions. Aloe vera medium was selected as the cultivation medium for EPS production by Bifidobacteria pseudocatenulatum ATCC 27919 due to it being rich with carbon sources. The aim of this study was to a) determine the cytotoxicity effect of EPS produced by B. Pseudocatenulatum strain ATCC 27919 cultivated in aloe vera medium against Caco-2 cell line using MTS assay, and b) study the component profile of EPS produced by B. pseudocatenulatum strain ATCC 27919 using CHNS elemental analysis, high-performance liquid chromatography (HPLC), flourier-transform infrared (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy techniques. The EPS obtained from aloe vera medium was found to be toxic to Caco-2 cells at concentration of 1 mg/ml at both 20- and 40-hour incubation with percentage cell viability of 82% and 65%, respectively. The cytotoxic activity of EPS was justified by determining the presence of mannose and molecular properties of EPS through CHNS, HPLC, FTIR and NMR analyses. As a conclusion, EPS produced by B. Pseudocatenulatum strain ATCC 27919 during cultivation in aloe vera medium has the potential to act as a cytotoxic compound against Caco-2 cells due to its biological components.
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