Anti-inflammatory Effects of Chrysanthmi Flos Extract
2022
Park, B.J. | Kil, K.J.
BACKGROUND: The anti-inflammatory effect of Chrysanthmi Flos (CF), primarily produced in the wild and recently being cultivated in Korea was investigated for using it as an anti-inflammatory material. METHODS AND RESULTS: For measuring anti-inflammatory activity, CF was freeze-dried (CFEFD) after water extraction. The production of nitric oxide (NO), prostaglandin E₂ (PGE₂) and proinflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] via ELISA, and expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), c-Jun N-terminal kinase (JNK)1/2, extracellular-signal-regulated kinase (ERK)1/2, p38, phosphorylated inhibitor of the transcriptional factor NF-κB (IκBα), p65 (a subunit of nuclear factor-κB) protein were determined using Western blot. The production of NO, PGE2, TNF-α, IL-1β, and IL-6 reduced in a concentration-dependent manner by CFEFD treatment (100 μg/ml - 400 μg/ml) than that by lipopolysaccharide treatment. iNOS and COX-2 protein expressions were inhibited at a concentration of 100 μg/ml - 400 μg/ml and 100 μg/ml - 200 μg/ml, respectively, by the CFEFD treatment. JNK1/2, ERK1/2, p38 and phosphorylated IκBα protein expressions were inhibited in a concentration-dependent manner by the CFEFD treatment. The expression of p65 protein increased in the cytoplasm and was inhibited in the cell nucleus in a concentration-dependent manner. CONCLUSIONS: Water extract of CF exhibited high anti-inflammatory activity, thus having high value as an anti-inflammatory material.
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