Identification of QTLS [Quantitative trait loci] governing sheath blight resistance in early-backcross breeding populations using SNP [single-nucleotide polymorphism] molecular markers
2024
Shrestha, U.
Sheath blight, caused by Phizoctonia solani, is economically important in rice production. The development of resistant elite breeding lines is cost-effective and efficient, but no known rice genotype exhibits complete resistance. This study, assessed the potential of eighty-two early-backcross selective introgression lines (EBSILs) derived from BG 300, Y134, and Khazar as donor parents and WTR 1 as the recipient parent to sheath blight disease resistance. The evaluation was conducted through artificial inoculation in pot and tray experiment under controlled greenhouse conditions. The genotyping of these (EBSILs) was performed using single nucleotide polymorphism (SNP) markers. In this study, nine EBSILs exhibited moderate resistance against sheath blight rice in and shower lower Area under the disease progress curve (AUDPC) values than the resistant check Teqing in both experiments. Days at flowering from seedling was negatively correlated with almost all traits and AUDPC was found to be positively correlated with relative lesion height. Thirty four QTLs were detected on chromosome 1, 2, 4, 5, 6, 7, 8, 9, 11 and 12 at 0. 01 significance level with an LOD value, -long p(F) greater than or equal to 4.75. Phenotypic variance explained (PVE) ranged from 4.15-27.64%. One major QTLs qTrLN12 for lesion number was identified on chromosome 12 and other 13 major QTL for morphological traits were identified with PVE value of more than 10%. Posible candidate genes associated with QTLs associated with sheath blight disease were also identified. However, these QTLs need further verification before utilizing them in the breeding program.
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